Experimental Approach in Molecular Biology and Biochemistry

课程简介

/ Indicative Syllabus Students will be presented with a problem or task that requires the use of at least the following molecular biology and biochemical techniques: (a) Site-directed mutagenesis using PCR, restriction digestion, plasmid cloning vectors; (b) Transformation into bacterial cells for amplification and purification from bacterial cells; (c) Protein expression, extraction, purification and characterizations; (d) DNA gel electrophoresis, Protein SDS-PAGE, DNA sequencing; (e) Study of protein modification and its implications Students will be asked to plan experiments and present it in the form of a proposal. Students will carry out the experiments involving the above techniques and collect data from their work. Students will meet tutors frequently to discuss the experiment progress and finalize details of coming laboratory work. Students will write up a report to present their work and findings. Students will present a scientific article in front of the whole class.

目标

This subject is a laboratory-based subject which will provide students a hands- on experience of designing, performing, and analyzing results from a molecular biology/biochemical mini-project.

先修要求

DNA Technology, Biochemical Techniques

Teaching Pattern

Methodology No formal lectures will be provided but a briefing session on the requirement of this subject and the