Protein Biotechnology

课程简介

/ Indicative Syllabus Protein purification (Micro to Macro): classical and modern techniques in protein purification; micropurification and analysis; purification of synthetic proteins; bulk precipitation and phase partition methods; chromatographic techniques in processing scale; FPLC system; new separation concepts. Protein folding: basic concepts; protein folding and diseases; protein-folding models; experimental approaches; crystallographic determination of protein structure. Protein engineering: site-directed mutagenesis and its application to protein structure; dissection and engineering of sites; development and testing of structural models. Protein production: production of secreted proteins in bacteria and yeast; expression of cloned genes in mammalian cells; Bacillus as an expression system; production of pharmaceutical proteins in the milk of transgenic animals; production of useful protein in transgenic plants; large scale culture of mammalian cell for production of therapeutic proteins. Industrial uses of enzymes: enzymes in food biotechnology and industry; enzymes in organic synthesis; enzymes in analysis and medicines; enzymes in genetic engineering. -- 1 of 3 --

目标

To understand the concepts in protein biotechnology, including purification of proteins, protein folding mechanisms and protein engineering. To introduce protein production and application of enzymes in biotechnology industries.

先修要求

Biochemistry, DNA Technology & Biochemical Techniques

Teaching Pattern

Methodology The basic concepts and knowledge will be presented and explained to students in lectures. A lab quiz will be given to students before the experiments in order to facilitate students’ active learning in laboratory sessions. Through the lab classes, students will gain hands-on experience on common protein techniques. They will also be able to enhance their learning of the principles through the experiments. In tutorials, short exercises and discussions will be used to gauge their learning outcomes, to supplement, and to reinforce their learning. Students will be encouraged to further explore in depth through study aids available on the internet and in the library.